Flow Cytometry is one of the most powerful single cell analysis tool used in biological research and clinical diagnostics. Using this state-of-the art technology, we can study and quantify various parameters of the cells or cell like particles in heterogeneous samples. In the last few years, the number of parameters (colors) simultaneously used in flow cytometry experiments has increased. This is enabled by the availability of high-end instruments and powerful data analysis tools. To harness the real power of multicolor flow cytometry, appropriate experimental designing, acquisition, data analysis and presentation is crucial.

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