Polymerase Chain Reaction (PCR) is a revolutionary molecular biology technique used to amplify specific DNA sequences, enabling the production of millions to billions of copies of a particular DNA segment. Developed by Kary Mullis in 1983, PCR has become an indispensable tool in various fields such as genetics, biotechnology, forensic science, and medical diagnostics. This comprehensive online PCR course is designed for students, researchers, and professionals in the fields of biology, genetics, biotechnology, clinical diagnostics and will cover basics of PCR and its applications, primer designing and reaction set up, different types of PCR (e.g., standard PCR, quantitative PCR & droplet digital PCR), PCR components and their roles, troubleshooting & common PCR issues and Q & A.

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