Flow Cytometry is one of the most powerful single cell analysis tool used in biological research and clinical diagnostics. Using this state-of-the art technology, we can study and quantify various parameters of the cells or cell like particles in heterogeneous samples. This 2nd Online International Winter School for Flow Cytometry Data Analysis & Presentation is a comprehensive program designed to provide participants with in-depth knowledge and practical skills in analyzing and presenting flow cytometry data. This course is ideal for researchers, students, and clinicians looking to enhance their expertise in this critical area of biological research and clinical diagnostics. Through lectures and practical activities, participants will learn the core concepts of flow cytometry data analysis & presentation and troubleshooting. We will cover the theory combining with data analysis sessions using different data sets as part of our course curriculum.